ISO 21528-1:2004 PDF Download

ISO 21528-1:2004 is a standard that outlines a method for the detection and enumeration of Enterobacteriaceae, a group of bacteria commonly found in food and animal feeding stuffs. The method described in this standard involves a pre-enrichment step, which involves incubating the sample in a liquid medium at 37 °C.

Enumeration of Enterobacteriaceae is carried out using the most probable number (MPN) technique, which involves calculating the estimated number of bacteria present in the sample based on the observed number of positive and negative tubes after incubation.

This method is suitable for samples where the target microorganisms are expected to require resuscitation before enrichment and when the expected number of bacteria is between 1 and 100 per milliliter or per gram of the sample.

It's important to note that this method has limitations and can exhibit a large degree of variability, which may impact the reliability and accuracy of the results.