ISO 23418:2022 PDF Download

ISO 23418:2022 is a standard that provides general requirements and guidance for the use of whole genome sequencing (WGS) in the typing and genomic characterization of bacteria in the food chain. The standard outlines the minimum requirements for the different stages involved in the WGS process, including the handling of bacterial cultures, isolation of genomic DNA, library preparation, sequencing, and assessment of raw DNA sequence read quality.

Furthermore, the standard covers the bioinformatics analysis required to determine genetic relatedness, genetic content, and the prediction of phenotype. It also includes guidelines for the capture of metadata and the deposition of sequence data in repositories. Additionally, the document emphasizes the importance of validating the entire WGS workflow to ensure it is fit for the intended application.

ISO 23418:2022 is applicable to bacteria isolated from various sources in the food chain, including products intended for human consumption, animal feed, environmental samples from food and feed handling and production areas, as well as samples from the primary production stage. The standard seeks to provide a standardized approach to WGS in the field of microbiology and enhance the understanding and control of bacterial strains in the food chain.